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OBJECTIVE:To investigate the effects ofAlisma orientalis polysaccharides on the insulin resistance and lipid metabolism disorder in type 2 diabetes mellitus (T2DM) rats and its possible mechanism.METHODS:T2DM rat model was induced by high fat diet combined with intraperitoneal injection of streptozotocin (30 mg/kg).50 model rats were randomly divided into model group (normal saline),glibenclamide group (positive control,25 mg/kg) and A.orientalis polysaccharide high-dose,medium-dose and low-dose groups (400,200,100 mg/kg),with 10 rats in each group.Other 10 normal rats were included in normal control group (normal saline).All groups were given drugs ig,once a day,for consecutive 6 weeks.After 5 weeks of administration,the glucose tolerance test was conducted.Fasting blood glucose (FBG) levels were measured in rats 0,30,60,90,120 min after intraperitoneal injection of 2 g/kg Glucose solution,respectively.After 6 weeks of administration,serum levels of FINS and FBG were measured,and HOMA-IR and insulin sensitivity index (ISI) were calculated;serum levels of FFA,LDL-C,HDL-C,TG and TC were detected,and the levels of SOD,GSH-Px and MDA in liver tissue were detected.RESULTS:Compared with normal control group,FBG levels of rats in model group at 0-120 min were increased significantly in glucose tolerance test.After 6 weeks of administration,the serum levels of FINS,LDL-C and ISI,and the levels of SOD and GSH-Px in the liver tissue of the model group were decreased,and the differences were statistically significant (P＜0.05).Compared with model group,each index of other administration group were improved significantly (P＜0.05),except that HOMA-IR,the serum levels of LDL-C and HDL-C were not significantly improved in A.orientalis polysaccharide low-dose group (P＞0.05).CONCLUSIONS:A.orientalis polysaccharide can improve insulin resistance and lipid metabolism disorder in T2DM rats through antioxidant effect.
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Objective: To investigate the characteristics of lower respiratory tract infection caused by Chryseobacterium and its antimicrobial susceptibility in vitro . Methods: The clinical data of 52 cases of lower respiratory tract infection caused by Chryseobacterium were analyzed, and the antimicrobial susceptibility of commonly used agents was determined with the method of agar dilution. Also the 50% lethal dosage (LD 50 ) (as the marker of virulence) of 20 randomly selected Chryseobacterium strains for mice were determined. Results: (1) Thirty six was over 60 years old;all of 52 cases had underlying diseases, mainly were chronic obstruction pulmonary disease and malignant tumors. Seventeen cases had the history of incubation or tracheotomy for mechanical ventilation, and 35 had history of broad antibiotics treatment. The mean hospitalization time before infection were 35.6 d, and 38.5% of the cases had mixed infection with other bacteria. No specific clinical manifestations and chest X ray appearance revealed. (2) The in vitro activity of 25 agents showed that these strains were highly resistant. (3) The range of the LD 50 of tested strains was 4.11?10 6 5.68?10 8/mouse, suggesting low virulence of this kind of bacteria. Conclusion: The lower respiratory tract infection caused by Chryseobacterium has no unique features; the incidence of the infection increases in immunosuppressed old patients with various underlying diseases, although the virulence is relatively low. Because the clinical isolates are highly resistant, the antibiotics should be selected according to the results of bacterial sensitivity test.
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To study the treatment of metastasis of experimental lung cancer with a recombinant adenovirus carrying IL 2 gene, concentrations of IL 2 and associated cytokines in murine lung lavage and blood were determined by ELISA at different times after intratracheal injection of the recombinant adenovirus. The lung metastasis nodes, survival time, survival rate were investigated in a C57BL/6 mouse model by intratracheal administration, and the NK activity and CTL activity were also determined by 51 Cr 4 hour release method. The results showed that IL 2 could be detected in lung tissue as early as 6 hours after administration of IL 2 gene, its concentration was higher in lung lavage than in peripheral blood, and was higher in experimental group than that in the control group; intratracheal use of the IL 2 carring recombinant adenovirus had significant therapeutic effect on metastasis of experimental lung cancer. Increased CTL and NK activities, longer survival time, and higher survival rate were observed in the experimental group compared with the control group. It is suggested that intratracheal usage of adenovirus vector carrying IL 2 gene might play therapeutic role on the lung cancer metastasis, indicating that gene therapy of lung tumors could be done through airway directly with recombinant adenovirus.
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Objective:To investigate the preventive and therapeutic effects of intraperitoneal injectoin of IFN? on bronchial asthma in mice and the relevant mechanism. Methods: Thirty-six BALB/c mice were randomly equalized into 3 groups:group A (normal control group),group B (asthmatic model group) and group C (IFN? treated group). The asthmatic model was established in group B and C by immunization with ovalbumin (OVA) absorbed to aluminum hydroxide. Mice of group B and C received 0.25 ml PBS and 5 ?g IFN? intraperitoneally on days 23 to 30 once daily prior to ovalbumin challenge,respectively. Bronchoalveolar lavage fluid (BALF) was collected on day 31 for determining the cellular composition and the concentrations of IL-4 and IL-5. Meanwhile,IgE in serum was determined. The pathological changes and the expression of GATA-3 were investigated in the lungs of mice. Results: (1) BALF eosinophils was significantly decreased in group C compared with those in group B ( vs ,P
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Objective: To investigate the effect of CpG motif oligodeoxynucleotides (CpG ODN) on the antigen induced allergic airway reaction in mice. Methods: The asthma model was set up in the C57BL/6 mice with OVA, the CpG ODN in the dose of 30 ?g was co administered intraperitoneally with the antigen in sensitization stage to study its effect on the airway allergenic reactions. Results: (1)Compared with the control, coadministration of CpG ODN in sensitization phase significantly inhibited airway eosinophilia after antigen challenge( P
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Objective:In order to study the antitumor immune response induced by antigen pulsed,IL 18 gene modified dendritic cells in vivo.Methods:①C57BL/6 mice were immunized twice subcutaneously by tumor antigen peptide pulsed,IL 18 gene modified dendritic cells(DC IL 18/mut1)(1?10 5 cells/mouse),then the NK activity and CTL activity were determined.②In block test,C57BL/6 mice were first immunized once 2?10 5 cells/mouse DC IL 18/mut1 subcutaneously,and then challenged by 5?10 5 3LL Lewis lung cancer cells/mouse with blocking different immune components by monoclonal antibody,the tumor growth were observed.Results:Specific CTL activity and NK activity could be induced most significantly in mice immunized with DC IL 18/mut1.The block test showed that CD4 +T,costimulating pathway,the production of IFN ? involved in the induction phase of immunization with DC IL 18/mut1,and CD8 +T?IFN ??NK cells involved in the effect phase but CD4 +T cells unnecessary.Conclusion:Immunization with DC msIL 18/mut1 could induce potent antitumor immune activity,whose mechanisms involved effective antigen presentation,increased CTL activity and NK activity,CD4 +?CD8 +T?NK cells incorporation,and the production of IFN ?.
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Objective: To study the treatment of experimental lung metastasis by intratracheal injection of IL 18 gene recombinant adenovirus. Methods: (1)The mouse IL 18 mRNA was detected by RT PCR, the concentrations of IL 18, associated cytokines in lung lavage and blood were determined by ELISA at different times after intratracheal injection of IL 18 recombinant adenovirus. (2)The lung metastasis nodes, mouse survival period, survival rates were investigated in the treatment of experimental lung metastasis in C57BL/6 mouse model. The NK activity and CTL activity were determined by 51 Cr 4 h release method. Results: (1)The IL 18 mRNA could be detected in lung tissue 6 h after intratracheal use of IL 18 recombinant adenovirus, and the concentrations of IL 18 in lung lavage was higher than that of peripheral blood, and both IL 18 mRNA and IL 18 could not be detected in control groups. (2)Intratracheal use of IL 18 recombinant adenovirus had significant therapeutic effect on experimental lung metastasis with the results of increased CTL and NK activity, and with longer survival period and higher survival rates compared with the control groups. Conclusion: Intratracheal usage of adenovirus vector containing IL 18 gene has therapeutic effect on the lung metastasis, denoting that gene therapy of lung diseases can be done through airway directly with recombinant adenovirus.
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Objective:To observe the expression of GATA-3in asthmatic mice lungs and to explore the feasibility of in-terleukin(IL-12)blockading GATA-3expression in treatment of bronchial asthma.Methods:C57BL/6mice were randomly divided into3groups:control group(group A),asthmatic model group(group B)and IL-12injection group(group C).Asth-matic models were established in group B and C.Normal saline(0.1ml)and IL-12(1?g)were injected in group B and C re-spectively on the1,3,7,9,25,26,27and28d.Six mice from each group were obtained for analyses of bronchoalveolar lavage fluid(BALF)on d31.The airway pathology changes and the expression of GATA-3were observed by hematoxylin/eosin(H-E)and immunohistochemistry respectively.Results:There was no symptom in group A,and the symptoms of group B were more severe than those of group C.Eosinophil(EOS)was not seen in the BALF of group A,while EOS in group B and group C were(20.0?4.0)%and(0.2?0.1)%respectively.In the same microscopic visual field,there was no inflam-mation cell in group A and a large number of inflammation cells in group B,but inflammation cells in group C were signifi-cantly decreased.Immunohistochemistry showed that the expression of GATA-3in group B was strong and no GATA expres-sion was found in group A and group C.Conclusion:The expression of GATA-3in asthmatic mice is high;IL-12can inhibit asthmatic airway and lungs inflammtion,whose mechanism may be the blockade of GATA-3expression in asthmatic models.[
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Objective To investigate the inhibitory effects of recombinant T-bet adenovirus (AdT-bet) on airway inflammation in mice with asthma, and to explore the relevant mechanisms. Methods C57BL/6 mice were randomly allocated into 4 groups with 12 mice each. The asthmatic model was reproduced in group A, B and C by administration of ovalbumin (OVA) in aluminum hydroxide. Mice in group A, B and C received 50?l AdT-bet (108pfu) and AdLacZ and PBS, respectively, 19 days prior to OVA challenge. Bronchoalveolar lavage fluid (BALF) was collected on the 26th day after the induction of asthma for the determination of the cellular composition and concentrations of IL-4, IL-5 and IFN ?. Meanwhile, IgE content in serum was determined. The pathological changes and the expression of GATA-3 in the lungs of these mice were investigated. Results The percentage of eosinophils (EOS) and concentrations of IL-4 and IL-5 in BALF of group A were 0.6%?0.2%, 6.8?3.7pg/ml, 12.5?4.8pg/ml, respectively, which were significantly lower than those in group B (20.9%?6.8%, 92.4?23.0pg/ml, 56.5?11.8pg/ml) and group C (20.8%?6.7%, 90.4?22.8pg/ml, 57.3?12.2pg/ml, P
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Objective To investigate the role of IFN-? in prevention and treatment of bronchial asthma and the mechanism of its effect. Methods BALB/C mice were randomly divided into three groups: group A (control group, n=12); group B (asthma model group, n=12); group C (IFN-? intraperitoneal treatment group, n=12). The asthma model was reproduced in group B and C with ovalbumin (OVA) adsorbed to aluminum hydroxide. PBS (0.25ml) and IFN-? 5?g was respectively injected intraperitoneally in group B and C on days 23 to 30 once daily prior to ovalbumin challenge. Bronchoalveolar lavage fluid (BALF) was collected on day 31 and its cellular composition was analyzed. The levels of IL-4, IL-5 and the IgE in serum were determined. The pathological changes in the lung and the expression of GATA-3 were observed. Results A notable decrease of eosinophils (0.3?0.2) in BALF was found in group C comparing with the group B (21.1?6.7) (P